Table 1. Enzymatic activities of wild-type and mutant derivatives of F29 DNA polymerase^a.

Parameter assayed	Substrate(s)	F29 DNA polymeraseb
		wt	T59F	Y59L	H59R	H61L	F69Y	F69S
F29 TP-DNA replication	F29 TP-DNA, dNTP	100	79	34	<1	20	58	45
F29 TP-DNA amplification	F29 TP-DNA dNTP	100	91	3	<1	1	39	4
Primed M13 DNA replication	Primed M13, dNTP	100	58	100	9	29	72	87
F29 TP-DNA initiation	F29 TP-DNA, TP, dATP	100	77	18	25	13	49	22
TP-deoxynucleotidylation	TP, dATP	100	102	19	10	4	43	7

^aThe different activity assays were carried out as described in Materials and Methods.

^bNumbers indicate the percentage of activity relative to the wild-type enzyme obtained from several experiments.