Skip to main content
. 2023 Apr 20;12(4):966. doi: 10.3390/antiox12040966

Figure 5.

Figure 5

Effects of EGCG on DRP1 and its S616 phosphorylation following SE. Western blot data showed that DRP1 expression and its S616 phosphorylation decreased in SE group, compared with control animals. In control animals, EGCG did not affect DRP1 expression and its S616 phosphorylation level. EGCG attenuated a decrease in DRP1 expression and its S616 phosphorylation in SE group (A–C). Immunofluorescent study also revealed that DRP1 expression was reduced in CA1 neurons of SE group, compared with control animals. In control animals, EGCG did not affect DRP1 expression in CA1 neurons. EGCG attenuated a decrease in DRP1 expression in CA1 neurons of SE group (D,E). (A) Representative Western blot of DRP1 and its S616 phosphorylation in the whole hippocampus. (B) Quantification of DRP1 protein level based on Western blot data (* and # p < 0.05 vs. control and vehicle-treated rats; n = 7 rats, respectively). (C) Quantification of DRP1 S616 level based on Western blot data (* and # p < 0.05 vs. control and vehicle-treated rats; n = 7 rats, respectively). (D) Representative photos of DRP1 expression and its intensity. (E) Quantification of DRP1 intensity in CA1 neurons (* and # p < 0.05 vs. control and vehicle-treated animals; n = 35 cells in 7 rats, respectively).