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. 2023 Apr 20;12(4):966. doi: 10.3390/antiox12040966

Figure 6.

Figure 6

Effects of EGCG on ERK1/2 and JNK activities following SE. Western blot data showed that ERK1/2 and JNK phosphorylation decreased in SE group without affecting their total protein levels. In control animals, EGCG did not affect ERK1/2 and JNK phosphorylation. EGCG attenuated decreased ERK1/2 but not JNK phosphorylation in SE group (A–C). Immunofluorescent study revealed that ERK1/2 phosphorylation was reduced in CA1 neurons of SE group, compared with control animals. In control animals, EGCG did not affect ERK1/2 phosphorylation in CA1 neurons. EGCG attenuated a decrease in ERK1/2 phosphorylation in the CA1 neurons (D,E) of SE group. (A) Representative Western blot of ERK1/2, JNK and their phosphorylation in the whole hippocampus. (B) Quantification of ERK phosphorylation level based on Western blot data (* and # p < 0.05 vs. control and vehicle-treated rats; n = 7 rats, respectively). (C) Quantification of JNK phosphorylation level based on Western blot data (* p < 0.05 vs. control rats; n = 7 rats, respectively). (D) Representative photos of phospho (p)-ERK1/2 level and its intensity. (E) Quantification of p-ERK1/2 intensity in CA1 neurons (* and # p < 0.05 vs. control and vehicle-treated animals; n = 35 cells in 7 rats, respectively).