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. 2023 Mar 30;13(4):623. doi: 10.3390/biom13040623

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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SPE-38::wrmScarlet-I visualized by red fluorescent signal localizes to fused MOs, the cell body and pseudopod plasma membranes in spermatozoa. (A–C) Imaging of the same in vivo activated control spermatozoa dissected from mated hermaphrodites. (A) Single confocal section of control spermatozoa. (B) Bright field image of control spermatozoa. (C) Merge of (A,B). (D–F) Imaging of spe-38::wrmScarlet-I; him-5(e1490) in vivo activated spermatozoa dissected from mated hermaphrodites. (D) Single confocal section of spe-38::wrmScarlet-I; him-5(e1490) spermatozoa. White arrow indicates fused MOs in a spermatozoa. (E) Bright field image of spe-38::wrmScarlet-I; him-5(e1490) spermatozoa. (F) Merge of (D,E). Yellow scale bars, 5 µm.