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. 2023 Apr 13;12(4):923. doi: 10.3390/antiox12040923

Figure 4.

Figure 4

The interaction of KDELC2 with the activation of NLRP3 inflammasome and autophagy in glioblastoma cells. (A) The decrease in NLRP3, caspase-1, and IL-1β protein expression after shKDELC2-transfected GBM8401 and U87MG. (B) Western blot analysis revealed decreased expression of LC3β in shKDELC2-transfected GBM8401 and U87MG. (C) KDELC2 knockdown glioblastoma led to a relatively lower mRNA expression of LC3β, STSQM1 (p62), and ATG4B. (D) The administration of NAC in glioblastoma cells inhibited the activated caspase-1, IL-1β, LC3β, and p62 protein expression. (E) The suppression of HUVEC proliferation was detected in glioblastoma cells after the use of 3MA and MCC950. (F) The application of 3MA, MCC950, and Mito-tempo inhibited FGF and VEGF expression in T98 and THP-1 co-cultured with T98-CM. Bars, means ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001.