Figure 3.

Indirect end labeling of the cyd1 region. (A) Restriction map of the cyd1 region. H, HindIII; D, DpnI; N, NdeII; N/D; partially methylated site at which molecules that are methylated on both strands are digested by DpnI and unmethylated molecules are digested by NdeII. Bars indicate probes for the experiments shown in (B) and (C). (B) Indirect end labeling experiment of the cyd1 region in DNA digested with HindIII and hybridized with probe b. Arrangement of the lanes and labeling is as in Figure 2B. The larger band in lane 4 is due to partial digestion of the DNA in this lane with HindIII. (C) Indirect end-labeling of the cyd1 region in DNA digested with PstI and hybridized with probe c. The numbers to the right of (B) and (C) indicate the size of the restriction fragments in lanes 3 and 7.