Figure 3.

mRNA expressions of CGRP (A) and inflammatory cytokines, including IL-1β (B) TNF-α (C), and IL-6 (D), in TNC in the five groups of rats as detected by RT-PCR (n = 3–4/group). Repeated IS infusions into dura increased the mRNA expressions of CGRP and the above inflammatory cytokines, while EA decreased mRNA levels of CGRP and the above inflammatory cytokines. ANOVA and Tukey’s post hoc analysis test were performed and data are presented as the mean ± standard deviation. * p < 0.05, ** p < 0.01, *** p < 0.001, Blank vs. IS; ⏀⏀ p < 0.01, ⏀⏀⏀ p < 0.001, PBS vs. IS; # p < 0.05, ### p < 0.001, #### p < 0.0001, Blank vs. SEA; + p < 0.05, +++ p < 0.001, ++++ p < 0.0001, PBS vs. SEA; ^ p < 0.05, ^^ p < 0.01, IS vs. EA; p = 0.1613 (CGRP), † p < 0.05, †† p < 0.01, SEA vs. EA.