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. 2023 Mar 24;13(4):541. doi: 10.3390/brainsci13040541

Figure 6.

Figure 6

Ibal-1, TLR4, and NF-κB protein expression levels in TNC assessed by Western blot assay in five groups of rats (n = 3/group). Repeated IS infusions into dura induced an increased protein expression of Ibal-1, TLR4, and NF-κB, while EA decreased protein expression of the above factors. (A) Ibal-1 protein expression levels. (B) TLR4 protein expression levels. (C) NF-κB protein expression levels. Protein expressions levels were normalised against β-actin and analysed using ANOVA and Tukey’s post hoc analysis test, and densitometry results are shown as the mean ± standard deviation. * p < 0.05, ** p < 0.01, Blank vs. IS; ⏀ p < 0.05, ⏀⏀ p < 0.01, PBS vs. IS; # p < 0.05, ## p < 0.01, Blank vs. SEA; + p < 0.05, ++ p < 0.01, PBS vs. SEA; ^ p < 0.05, ^^ p < 0.01, IS vs. EA; † p < 0.05, †† p < 0.01, ††† p < 0.001, SEA vs. EA.