Table 2.
Antibodies used in immunohistochemistry and Western blot analysis.
| Antibody | Manufacturer | Catalogue Number | Host | Dilution |
|---|---|---|---|---|
| For immunohistochemistry | ||||
| Ibal-1 | Abcam, Cambridge, UK | ab178847 | Rabbit | 1:200 |
| IL-1β | Abcam, Cambridge, UK | ab254360 | Rabbit | 1:50 |
| TNF-α | Bioss, Beijing, China | bs-2081R | Rabbit | 1:100 |
| IL-6 | Bioss, Beijing, China | bs-6309R | Rabbit | 1:100 |
| Anti-Rabbit IgG (HRP) | AiFang, Changsha, China | AFIHC003 | Goat | 1:200 |
| For Western blot analysis | ||||
| Ibal-1 | Abcam, Cambridge, UK | ab178847 | Rabbit | 1:1000 |
| TLR4 | Novus, St Charles, MO, USA | NB100-56566 | Mouse | 1:1000 |
| NF-κB | CST, Danvers, MA, USA | 6956T | Mouse | 1:1000 |
| β-actin | Zenbio, Chengdu, China | 200068-8F10 | Mouse | 1:10,000 |
| Anti-Rabbit IgG (HRP) | Zenbio, Chengdu, China | 511203 | Goat | 1:10,000 |
| Anti-Mouse IgG (HRP) | Zenbio, Chengdu, China | 511103 | Goat | 1:10,000 |