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. 2023 Apr 8;12(8):1109. doi: 10.3390/cells12081109

Figure 3.

Figure 3

CV upregulates phosphorylation of PKC-δ in RANKL-stimulated OCs. (A) Amount of cAMP at 12 h after stimulation of CV (10 ng/mL). Results are shown as means ± SD. (B) Western blotting was performed on protein extracts from OC precursors stimulated with CV (10 ng/mL) for 0, 5, 10, 15, 30 and 60 min. Total amount of PKC-δ and p-PKC-δ was evaluated using the antibodies described in the Materials and Methods section. Densitometric analysis of p-PKC-δ signals is shown. The relative level of p-PKC-δ expression was normalized to total PKC-δ expression. All assays were performed in triplicate and the results were expressed as the mean ± SD. One representative picture band out of three experiments is shown. * p < 0.05.