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. 2023 Apr 7;12(8):1107. doi: 10.3390/cells12081107

Figure 2.

Figure 2

Patterns of Myomaker expression during pig skeletal muscle development. (A) Relative mRNA expression of Myomaker in representative tissues (Heart, Liver, Spleen, Lung, Kidney, Longissimus dorsi and Skin) of E85 pig, β-actin and GAPDH were used as reference genes; (B) Relative mRNA expression levels of Myomaker in pigs at different ages (E85: Embryonic Day 85; E113: Embryonic Day 113; D0: Day 0 of life; D180: Day 180 of life; D270: Day 180 of life; Y2: year 2 of life); (C–E) Protein levels of Myomaker at different tissues and ages were detected by Western blot and normalized to Tublin. (F) Relative mRNA expression levels of Myomaker during proliferation (GM: Growth medium, 50%) and differentiation (DM2: Differentiation culture for 2 days; DM4: Differetiation culture for 4 days; DM6: Differentiation culture for 6 days; DM8: Differentiation culture for 8 days) of porcine MSCs. (G) Immunofluorescence with an anti-Pax7 antibody. Red arrow, Pax7 specific positive cells in nucleus. Bar, 100 µm; (H) Relative mRNA expression levels of Pax7 in MSCs, IPEC, and PK15; (I) Phase-contrast micrographs of proliferation (GM, 50%) and differentiation (DM2, DM4, DM6, and DM8) of pig MSCs. Bar, 100 µm; Values in A and B are mean ± S.D. with six pigs per group. Values E and G are mean ± S.D. with three cultures per group. ns = not significant; * p < 0.05; ** p < 0.01 compared between corresponding groups.