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. 2023 Apr 7;12(8):1107. doi: 10.3390/cells12081107

Figure 3.

Figure 3

Myomaker plays an important role in pig myoblasts fusion. (A) Relative mRNA expression levels of Myomaker after transfection with pcDNA3.1-Myomaker or pcDNA3.1 empty vector at 48 h; (B) Immunostaining with anti-MyHC antibody was performed on MSCs after transfected with pcDNA3.1-Myomaker or pcDNA3.1-empty vector at 48 h. Bar, 50 µm; (C,D) The differentiation index and fusion index of MSCs after transfection was calculated; (E–G) Relative mRNA expression levels of MyoG, MyoD, and Myf5 after transfection. These data are expressed as mean ± S.D. of three independent experiments. ns = not significant; * p < 0.05; ** p < 0.01 compared with negative control.