Figure 3.

A3B is repressed during G₀/early G₁ arrest in normal-like MCF10A cells and A3B-high BT474 cells. (A) Palbociclib arrests MCF10A and BT474 cells in G₀/early G₁ by direct interference with the RB/E2F pathway. Cells were treated for 24 h to induce growth arrest, followed by washout and subsequent sampling. (B,C) Cell cycle progression (top panels) and RT-qPCR and immunoblot analysis (center and bottom panels, respectively) of A3B, Cyclin E2, and Cyclin B1 during and after palbociclib-induced growth arrest of MCF10A and B474 cells. For immunoblots, samples of both cell lines were loaded on the same gel, but data are depicted separately here due to image intensity adjustments required for visualization of A3B in each cell line. U = unsynchronized. Error bar represents SD. Data represent n = 4 independent experiments.