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. 2023 Apr 8;13(8):1375. doi: 10.3390/diagnostics13081375

Figure 2.

Figure 2

(A) Energy transfer between two fluorophore molecules. (B) Jablonski diagram for donor–acceptor pair in FRET. (C) Orientation of donor–acceptor pair, parallel to each other for maximum transfer of energy represented by spectral overlapping of emission spectra of donor (D) and absorption spectra of an acceptor (A). (D) Overlapping integral during FRET and (E) dipole–dipole orientation for maximum transfer of energy.