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. 2023 Apr 18;15(8):2363. doi: 10.3390/cancers15082363

Figure 4.

Figure 4

SSZ treatment increases the levels of ROS in hypoxic colorectal cancer cells, leading to DNA damage and radiosensitization. DLD-1 and HCT116 cells were treated (16 h) under hypoxic conditions (1%) with the indicated concentrations. When applicable, NAC was added 1 h prior to SSZ treatment. (A) Summarizing graph showing ROS levels in DLD-1 (top) and HCT116 (bottom) cell lines. (B) Survival fraction of cells after radiation with 8Gy in DLD-1 (top) and HCT116 (bottom) cell lines. (C) The amount of DNA double-strand breaks in DLD-1 (top) and HCT116 (bottom) cell lines. Data are represented as mean ± SEM, n ≥ 3. A one-way ANOVA followed by Dunnett’s multiple comparison test or a two-way ANOVA followed by Dunnett’s, or Bonferroni’s multiple comparison test was performed for statistical analyses. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.