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. 2023 Apr 18;15(8):2363. doi: 10.3390/cancers15082363

Figure 6.

Figure 6

SSZ radiosensitizes colorectal cancer spheroids in a similar manner observed in 2D cultures. DLD-1 and HCT116 3D models were treated with SSZ (16 h) and irradiated with 8 Gy. (A) The growth of the spheroids was followed up by the EVOS after SSZ treatment (16 h) in DLD-1 (left) and HCT116 (right) 3D models under hypoxic conditions (0.1%). (B) Spheroid growth was followed up after SSZ treatment (16 h) and irradiation with 8 Gy in DLD-1 (left) and in HCT116 (right). (C) Representative pictures of the levels of ROS under hypoxic conditions (1%) in DLD-1 (top) and in HCT116 (bottom). (D) Summarizing graphs showing the mean gray values in DLD-1 (left) and HCT116 (right). (E) Representative pictures of the amount of lipid peroxidation after SSZ treatment under hypoxic conditions (1%) in DLD-1 (top) and in HCT116 (bottom). (F) Summarizing graphs showing the mean gray values in DLD-1 (left) and HCT116 (right). Data represented as mean ± SEM, n ≥ 3. A one-way ANOVA followed by Dunnett’s multiple comparison test was performed for statistical analysis. * p < 0.05, ** p < 0.01, *** p < 0.001.