Figure 13.

Interaction of ddFKBP::UL53 fusion proteins with pUL50-HA. 293T cells were transiently transfected with either the constructs coding for the three different ddFKBP::UL53 versions alone or in combination with pUL50-HA. Coexpression of the original core NEC heterodimer, pUL50-HA + pUL53-Flag, was used as a positive control; RFP + vector (pcDNA3.1) served as a negative control. To investigate protein stability, each fusion protein was expressed in the presence or in the absence of Shield-1 (1 µM). At 2 d p.t., cells were lysed, lysate controls were taken, and pUL50-HA was immunoprecipitated using mAb-HA. CoIP samples, intended to demonstrate NEC-specific protein interaction, were subjected to standard Wb analysis using specific antibodies as indicated.