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. 2023 Apr 19;12(8):1189. doi: 10.3390/cells12081189

Figure 2.

Figure 2

Mild heat shock inhibits membrane permeability. (A) unstimulated controls show no increased permeability: iBMMs were incubated at 37 °C (black circles) or exposed to 39 °C for one hour (red squares) followed by addition of PI (5 μM) to assay membrane permeability over time. (B) LPS controls show no increased permeability: iBMMs were primed with LPS and incubated at 37 °C for the entire 4 hours (black circles) or switched to 39 °C for the last hour (red squares), followed by addition of PI. (C) LPS+ATP stimulation shows that exposure to 39 °C inhibits ATP-induced pore formation in the membrane in LPS-primed cells: iBMMs were primed with LPS and incubated at 37 °C for the entire 4 hours (black circles) or switched to 39 °C for the last hour (red squares), followed by addition of ATP + PI The results are representative of two independent experiments. **** p < 0.0001 as determined by a two-way ANOVA with Tukey’s multicorrection comparison.