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Effect of mannose and 5-FU single and combination treatments on reactive oxygen species (ROS) production in human CRC cells. (A) HCT116, (B) HCT116 p53−/−, and (C) HCT116-5FUR cells were treated with mannose (man 50 mM) and/or 5-FU (5 µM) for 24 h. ROS levels represent an average of three independent experiments ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001 are considered statistically significant.
