Figure 11.

Migratory and invasive potential of 84E7 cells with AR activation: (A) Representative images of migrated 84E7 cells in the presence and absence of AR activation at 10× magnification. (B) Quantification of migrated cells. A six-fold increase in migration was observed in DHT-treated cells. (C) Representative images of 84E7 cells invading through the lower surface of the Matrigel insert membrane at 10× magnification. (D) Invasion index of EtOH- and DHT-treated 84E7 cells. Invasion index is calculated as mean number of cells invading the Matrigel insert membrane divided by the mean number of cells migrating through the control insert membrane ×100. EtOH-treated = 30.38; DHT-treated = 26.09. No significant change in the invasion potential of AR-induced senescent cells (* p-value < 0.05, ns = not significant).