Figure 5.

Pre- or post-stimulation of macrophages with IL-4 and IFNγ impacts the amount of S.tm phagocytosed by F4/80⁺CD11b⁺ macrophages. Unpolarised BMDM and pre-stimulated polarised BMDM (2 ng/mL IL-4 or 20 ng/mL IFNγ) were infected with S.tm expressing RFP (STR) for 1 h. Unpolarised cells were then stimulated (post-stimulation) with 10 ng/mL IL-4, 100 ng/mL IFNγ or left unstimulated for 4 h. Percentages of ARG1, iNOS and STR in macrophages were analysed via flow cytometry. (a) Percentage of ARG1-positive cells in F4/80⁺CD11b⁺ cells in pre-stimulated compared to post-stimulated BMDM. Data shown for uninfected (left) and infected (right) cells. (b) Percentage of iNOS positive cells in F4/80⁺CD11b⁺ in pre-stimulated compared to post-stimulated BMDM. Data shown for uninfected (left) and infected (right) cells. (c) Percentage of STR positive cells in F4/80⁺CD11b⁺ cells in infected pre-stimulated compared to post-stimulated BMDM. Statistical significance was determined by one-way ANOVA with Tukey post hoc test. * p-value < 0.05; *** p-value < 0.001. Representative data (mean ± SEM) from three independent experiments with two technical replications are shown. Data were normalised to the unstimulated uninfected Ctrl or, as indicated, to the unstimulated infected Ctrl.