Figure 6.

Different multiplicities of infection do not alter the effects of IL-4 and IFNγ stimulation on the growth of S.tm in macrophages. Unpolarised BMDM were infected with S.tm for 1 h. Afterwards, cells were stimulated (post-stimulation) with 10 ng/mL IL-4, 100 ng/mL IFNγ or left unstimulated as indicated. (a) CFU analysis after 0.5 h (a), 4 h (b) and 24 h (c) of infection with several multiplicities of infection (MOI) of S.tm in post-stimulated BMDM. Statistical significance was determined by one-way ANOVA with Tukey post hoc test: * p-value < 0.05; ** p-value < 0.01; *** p-value < 0.001. Representative data (mean ± SEM) from three independent experiments with 2–3 technical replications are shown.