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. 2023 Apr 14;12(8):1164. doi: 10.3390/cells12081164

Figure 9.

Figure 9

IL-4 exerts its inhibitory effect on S.tm proliferation via L-arginine-mediated metabolic pathways. Effects of L-arginine depletion and deletion of ARG1 on S.tm survival in macrophages was analysed. BMDM generated from C57BL/6N mice were cultured in L-arginine-free medium. Moreover, BMDM of Tie2Cre+/−ARG1fl/fl (KO) and Tie2Cre−/−ARG1fl/fl (WT) were generated. Macrophages were infected with S.tm and stimulated (post-stimulation) with 10 ng/mL IL-4, 100 ng/mL IFNγ or left unstimulated as indicated. (a) Effect of arginine-depleted medium (− L-Arg) on survival of S.tm in BMDM compared to S.tm survival in medium supplemented with L-arginine (+ L-Arg). (b) S.tm CFU in Tie2Cre−/−ARG1fl/fl (WT) BMDM compared to Tie2Cre+/−ARG1fl/fl (KO) BMDM after post-stimulation as indicated. Statistical significance was determined by one-way ANOVA with Tukey post hoc test: * p-value < 0.05; ** p-value < 0.01; *** p-value < 0.0001. Representative data (mean ± SEM) of two to three independent experiments with 2–4 technical replications are shown.