Figure 3.

Overview of the bulk segregant RNA-Seq approach used to identify candidate genes for melanoid. (A) RNA was isolated for wild-type and melanoid siblings, and >30 m read pairs were generated for each pool. Sequences were aligned to the axolotl genome and used to identify a region of Chr14q that was differentially enriched for SNPs between the pools. (B) Map of a Chr14q genomic region showing the positions of Ltk and Gphn. There are at least 70 predicted genes within the interval defined by these two genes.