Figure 3.

QUE in combination with OXP synergistically increased the ROS level in HCT116 cells. HCT116 cells were plated in a black-bottom 96-well plate at a density of 1 × 10⁴ cells per well. After incubating for 24 h, cells were treated with 20 μM H₂DCFDA for 30 min, followed by incubation with QUE (25 μM), OXP (0.5 μM), both compounds and tBHP (100 μM or 200 μM, a positive control for ROS production) in the absence (A) or presence (B) of SFN for 30 min. Subsequently, the fluorescence was measured. Error bars, mean ± SEM (N = 3). Significant differences among the experimental groups are denoted by different alphabetical letters (p < 0.05, one-way ANOVA with Duncan’s post hoc HSD test).