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. 2023 Apr 27;18(4):e0284402. doi: 10.1371/journal.pone.0284402

Fig 1. The M28_Spy1638 gene encodes a FakB4 protein and belongs to the FabT regulon.

Fig 1

A) Phylogenetic tree of the three S. pneumoniae (Spneu), four S. suis (Ssuis), three S. pyogenes (Spyo), FakB proteins and the M28_Spy1638 product, named FakB4; the branch lengths are, from top to bottom, 0.17461, 0.15163, 0.20011, 0.13539, 0.13176, 0.17447, 0.15335, 0.1527, 0.18335, 0.22949, 0.21848. B) Sequence comparison of S. pyogenes and S. suis FakB4 proteins. C) Relative expression of fabT, green, and fakB4 orange, in the mFabT versus the WT strain. Strains were grown in THY or THY-Tween 80 medium and RNAs were quantified by qRT-PCR. Expression was normalized to that of gyrA; relative gene expression is expressed as the log2-fold ratio in the mFabT versus the WT strain. 2-way ANOVA, Bonferroni post-test, ****p<0.0001. D) Cartoon representing the regulatory region of the fakB4 gene with -35 and extended -10 boxes, previously published [18], FabT consensus sequence and fakB4 translation initiation site indicated in black, red and blue, respectively.