Table 1.
Kinetic constants for SIRT2, SIRT3, and SIRT5 for selected substrates (Figure 2 and Figure 3). SD denotes the standard deviation of three independent replicates, and n.d. denotes not determined.
| Enzyme | Substrate | KM ± SD (nM) | kcat ± SD × 103 (s−1) | kcat/KM (M−1s−1) |
|---|---|---|---|---|
| SIRT2 | F1 | 16 ± 3 | 8.4 ± 1.9 | 536,000 |
| F2 | 39 ± 5 | 6.7 ± 1.0 | 175,000 | |
| F4 | 33 ± 5 | 23 ± 3 | 697,000 | |
| F5 | 43 ± 5 | 15 ± 2 | 335,400 | |
| Mcm1 | 17 ± 2 | 6.8 ± 1.5 | 400,000 | |
| SIRT3 | F1 | >20 µM | >17 | n.d. |
| F2 | >20 µM | >6 | n.d. | |
| F4 | 530 ± 70 | 23 ± 6 | 44,000 | |
| F5 | 990 ± 70 | 27 ± 6 | 27,000 |