Figure 5.

ALA inhibits NF-κB, AP1, GZMA, and GZMB gene expression in γMtb-cultured NK cells. PBMCs of healthy LTBI+ donors were cultured with or without γMtb (10 μg/mL) and in the presence or absence of ALA, as mentioned in the Methods section. After 48 h, NK cells were isolated by flow sorting, RNA was collected, and real-time PCR analysis was performed to determine various signaling molecules and transcription factors. PBMCs from six LTBI+ donors were used for this experiment. Paired t-tests were used to compare the differences between untreated and treated PBMCs from the same samples. The significant p values are shown (p < 0.05); ns: not significant, p > 0.05.