Figure 8.

NTS immunoreactivity in offspring ovaries. Representative images of the ovary in the three experimental groups in the first three weeks of postnatal life showing the presence of NTS-ir in oocytes from primordial and primary follicles (arrowheads) (a–e,g) as well as in granulosa cells from multilayered primary (f,i) and secondary (h) follicles (black arrows) and in theca cells (red arrows). AS: antral space; GC: granulosa cells; Oc: oocyte; SF: secondary follicle; TC: theca cells. Scale bars: (a–e): 40 µm; (f): 12 µm; (g): 30 µm; (h): 15 µm; and (i): 10 µm.