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Representation of GnRH3 neurons development at 48 hpf (A). Confocal images of TUNEL assay on tg(GnRH3:EGFP) at 48 hpf in embryos treated with 1.2% DMSO, 5 and 50 nM BaP (B–D). Embryos were acquired mounting the heads in dorsal view and each experiment was performed in triplicate using about 30 embryos/stage.
