Figure 1.

RES alleviated the IFN response by poly I:C. (A) The expression of mtRNAs in NS-SV-AC cells upon poly I:C transfection. (B) Heatmap of mRNA-seq results upon poly I:C transfection with or without RES pretreatment for type I IFN genes. The two columns represent log₂ fold changes of two biological replicates. (C) The ratios of ISG induction upon poly I:C transfection between RES-pretreated and control samples. (D) The APH assay was used to measure the effect of RES pretreatment on cell viability. n = 4 and error bars are s.e.m. (E) The ratios of mtRNAs induction upon poly I:C transfection between RES-pretreated and control samples. For ratios, Cq values were first normalized to that of ACTB mRNA. For untreated samples, the values were normalized to RNAs from control cells without poly I:C transfection. Similarly, RNAs from RES-pretreated cells with poly I:C transfection were normalized separately from those from RES-treated cells without poly I:C. Unless mentioned otherwise, three independent experiments were carried out, and error bars denote s.e.m. All statistical significances were calculated using one-tailed Student’s t-tests; * p ≤ 0.05, ** p ≤ 0.01, and *** p ≤ 0.001.