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. 2023 Apr 17;24(8):7403. doi: 10.3390/ijms24087403

Figure 2.

Figure 2

RES pretreatment rescued SS-related protein expressions. (A) Representative images of ZO-1 and occludin upon poly I:C transfection with or without RES pretreatment. The quantification of ZO-1 and occludin expressions is presented as the mean fluorescence intensity per spheroid. Intensities were normalized to the data from each experimental group cultured without poly I:C. n = 3 and error bars are s.e.m. (B) Visualization of GFP-tagged AQP5 expression upon poly I:C transfection. The scale bar represents 100 μm. The mean fluorescence intensities were normalized to the data from each experimental group cultured without poly I:C. In the bar graphs, the “–“ sign indicates RES-untreated/Mock-transfected, whereas the “+” sign indicates RES-treated/poly I:C-transfected. n = 3 and error bars are s.e.m. All statistical significances were calculated using one-tailed Student’s t-tests; * p ≤ 0.05, ** p ≤ 0.01.