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. 2023 Apr 9;24(8):6972. doi: 10.3390/ijms24086972

Figure 5.

Figure 5

Ang II induces the secretion and synthesis of TGF-β1 and ET-1 via the AT1R/Gαq axis in adult HCFs. Serum-starved adult HCFs were incubated with valsartan (valsar; 1 µM), PD123319 (1 µM), FR900359 (Gαq inh; 1 µM), or LY2109761 (TGF-β inh; 5 µM) for 1 h before stimulation with 200 nM Ang II for 24 (A) or 6 h (B). (A) After Ang II treatment, the culture medium was collected. The amounts of TGF-β1 and ET-1 secreted into the culture medium were determined by ELISA. (B) Relative mRNA levels of TGF-β1 and ET-1 were analyzed by qRT-PCR. Data are expressed as the mean ± SEM (n = 4). * p < 0.05 vs. vehicle; # p < 0.05 vs. Ang II.