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. 2023 Apr 9;24(8):6972. doi: 10.3390/ijms24086972

Figure 7.

Figure 7

Restorative effects of ARB, TGF-β inhibitor, and ERA on Ang II-induced myofibroblast differentiation. (A) Experimental design and timeline of the treatment in cultured adult HCFs. (B,C) Fibroblasts were treated with 200 nM Ang II for 24 h to activate fibroblast-to-myofibroblast transformation. After 24 h, cells were replaced with media containing Ang II with different inhibitors, including valsartan, LY2109761, bosentan, and LY2109761 plus bosentan for up to 3 days. Analysis was performed on days 1 or 3 after cotreatment. At the end of treatment, immunofluorescence staining was used to determine α-SMA expression (green) (B) and stress fiber formation (red) (C). Nuclei were stained with DAPI (blue). Scale bar, 10 μm. Data are expressed as the mean ± SEM (n = 3). * p < 0.05 vs. vehicle; # p < 0.05 vs. Ang II.