Figure 2.

FAS cells up-regulate brain metastasis genes. (a) FAS cells showed enhanced expressions of brain metastasis-related genes. The gene expression was evaluated using qPCR. The statistics were calculated using two-way ANOVA with the post hoc Tukey test. n = 3. (b,c) The expressions of COX2 and SerpinB2 in all groups were tested using immunofluorescence staining. Scale bar = 10 μm. (d,e) Quantification of the fluorescence intensity of COX2 and SerpinB2 in (b,c). The statistics among three groups were calculated based on one-way ANOVA with the post hoc Bonferroni test. n = 50. (ns: no significance, * p < 0.05, *** p < 0.001, **** p < 0.0001).