Skip to main content
. 2023 Apr 11;24(8):7087. doi: 10.3390/ijms24087087

Figure 5.

Figure 5

The FAS cells up-regulate multiple adhesion molecules that are clinically relevant to breast cancer brain metastasis. (a) FAS cells had enhanced expressions of brain metastasis-related adhesion genes. qPCR was conducted to test the gene expression. The statistics were analyzed based on two-way ANOVA along with the post hoc Tukey test. n = 3. (b) The highly expressed adhesion molecules in the FAS cells were clinically related to brain metastasis. The gene expression data were collected from the GEO public database (GSE173661, n = 25 samples). The paired comparison was analyzed using Graphpad. For each gene, a paired Student’s t-test was used to analyze the difference between the statistics in two groups. (c,d) The expressions of MUC1 and VCAM1 were tested through immunofluorescence staining. Scale bar = 10 μm. (e,f) Quantification of the expressions of MUC1 and VCAM1 in (c,d). The statistics among three groups were calculated based on one-way ANOVA with the post hoc Bonferroni test. n = 50. All data are represented by mean ± SEM. (ns: no significance, **** p < 0.0001).