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. 2023 Apr 13;24(8):7195. doi: 10.3390/ijms24087195

Figure 1.

Figure 1

(A) Alkaline Comet assay measures of DNA damage formation (% Tail DNA) in whole-blood peripheral blood lymphocytes (WB-PBL) following either 20, 10, or 2 × 10 Gy conventional dose rate (CONV, 0.1 Gy/s) irradiation or 20 Gy FLASH (2 kGy/s), at either 0.5 or 21% oxygen tension, with select CONV and FLASH 0.5% O2-irradiated samples, then re-irradiated with 10 Gy CONV under normoxia. (B) Alkaline Comet assay measures of DNA damage formation (% Tail DNA) following treatment of WB-PBL samples with cisplatin (10–1000 µM) or control vehicle (0.9% saline solution) for 6 h at room temperature prior to 10 Gy 195 Kv X-ray irradiation at 21% O2. (C) The calculated ‘Percentage Decrease in % Tail DNA (%TD)’ (see Section 4), being indicative of cisplatin-induced crosslink formation. All data are expressed as the mean % Tail DNA of three slides (n = 3). Error bars indicate standard deviation of the means for each experimental condition. Statistical significance (two-tailed unpaired t-test) is indicated with *, where * is p < 0.05, ** is p < 0.01 and **** is p < 0.0001, non-significant (ns) is p > 0.05.