Table 1.
Gene Name | Official Symbol | Accession Number | Product Length | Forward Primer | Reverse Primer |
---|---|---|---|---|---|
GAPDH | GAPDH | NM_001256799.3 | 81 | CTGGTAAAGTGGATATTGTTGCCAT | TGGAATCATATTGGAACATGTAAACC |
ALP | ALPL | NM_001127501.4 | 86 | GGAACTCCTGACCCTTGACC | 5′TCCTGTTCAGCTCGTACTGC3′ |
OC | BGLAP | NM_001199662.1 | 102 | GGCAGCGAGGTAGTGAAGAG | CTCACACACCTCCCTCCTG |
ON | SPARC | NM_001309444.2 | 95 | GAGGAAACCGAAGAGGAGG | 5′GGGGTGTTGTTCTCATCCAG3′ |
RUNX2 | RUNX2 | NM_001015051.4 | 78 | GTAGATGGACCTCGGGAACC | 5′GAGGCGGTCAGAGAACAAAC3′ |
OPN | SPP1 | NM_000582.3 | 348 | GGTGATGTCCTCGTCTGTA | CCAAGTAAGTCCAACGAAAG |
COL1A1 | COL1A1 | NM_000088.4 | 52 | 5′GAGTGCTGTCCCGTCTGC3′ | 5′TTTCTTGGTCGGTGGGTG3′ |
GSK-3β | GSK3B | NM_001146156.2 | 106 | 5′GGAACTCCAACAAGGGAGCA3′ | 5′TTCGGGGTCGGAAGACCTTA3′ |
β-catenin | CTNNB1 | NM_001330729.2 | 108 | 5′ATGGAGCCGGACAGAAAAGC3′ | 5′CTTGCCACTCAGGGAAGGA3′ |
LEF1 | LEF1 | NM_001130713.3 | 134 | 5′CTTTATCCAGGCTGGTCTGC | 5′TCGTTTTCCACCATGTTTCA |
IHH | IHH | NM_001346281.2 | 109 | CAGCGATGTGCTCATTTTCCT | AAGGCTCTCAGCCTGTGAGG |
SMAD7 | SMAD7 | NM_005904.4 | 95 | CCCATCACCTTAGCCGACTC | TGGACAGTCTGCAGTTGGTTT |
VDR | VDR | NM_001017535.2 | 87 | CTCTGATAGCCTCATGCCAGG | ACCCAAAGGCTTCCCAAAGAG |
TGFB1 | TGFB1 | NM_000660.7 | 300 | 5′GAAACCCACAACGAAATC3′ | 5′AATTTCCCCTCCACGGCT3′ |
TNF | TNF | NM_000594.4 | 139 | 5′ACTTTGGAGTGATCGGCC3′ | 5′GCTTGAGGGTTTGCTACAAC3′ |