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. 2023 Apr 11;24(8):7044. doi: 10.3390/ijms24087044

Figure 4.

Figure 4

Immunostaining of interleukin (IL)-6 with zebrafish embryo and survivability after microinjection of apoA-I (28 ng) or CIGB-258 (1 ng) with CML (500 ng) and into zebrafish embryo at 1 h post-fertilization. (A) Immunofluorescence image of immunostained with anti-IL-6 antibody (ab208113, Abcam London, UK) as primary antibody (1:100 diluted) and goat anti-Rabbit IgG H&L (Alexa Fluor® 488, ab150077, Abcam, London, UK) as a secondary antibody (1:300 diluted) at 24 h post-injection. a. PBS alone; b. CML + PBS; c. CML + apoA-I; d. CML + CIGB-258. (B) Calculation of the immunostained fluorescence area (left axis) using Image J software version 1.53r (http://rsb.info.nih.gov/ij/ accessed on 16 January 2023) to convert the green intensity from the immunostaining. Survivability of the embryo at 24 h post-injection was also presented as the right axis. **, p < 0.01; ***, p < 0.001 of the immunofluorescence. #, p < 0.05 versus PBS + CML; ##, p < 0.01 versus PBS + CML of the survivability.