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. 2023 Apr 11;24(8):7044. doi: 10.3390/ijms24087044

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Histologic analysis of the hepatic tissue from zebrafish injected with PBS alone (photograph a), CML+ PBS (photograph b), CML+ Infliximab (photograph c), CML + Etanercept (photograph d), CML + Tocilizumab (photograph e), and CML + CIGB-258 (photograph f) under the consumption of a high cholesterol diet (HCD). (A) Photographs show the infiltration of neutrophils by Hematoxylin & Eosin (H&E) staining after converting the Hematoxylin-stained area into a red intensity. The yellow scale bar indicates 100 μm. The red box area in image of 400× magnification was observed again at 1000× magnification (B) Quantification of the nucleus area from the H&E staining using Image J software version 1.53r (http://rsb.info.nih.gov/ij/ accessed on 15 September 2022). The statistical significance of the groups was indicated as p-values at the top of the graph.

Histologic analysis of the hepatic tissue from zebrafish injected with PBS alone (photograph a), CML+ PBS (photograph b), CML+ Infliximab (photograph c), CML + Etanercept (photograph d), CML + Tocilizumab (photograph e), and CML + CIGB-258 (photograph f) under the consumption of a high cholesterol diet (HCD). (A) Photographs show the infiltration of neutrophils by Hematoxylin & Eosin (H&E) staining after converting the Hematoxylin-stained area into a red intensity. The yellow scale bar indicates 100 μm. The red box area in image of 400× magnification was observed again at 1000× magnification (B) Quantification of the nucleus area from the H&E staining using Image J software version 1.53r (http://rsb.info.nih.gov/ij/ accessed on 15 September 2022). The statistical significance of the groups was indicated as p-values at the top of the graph.