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Overview of non-viral transgene insertion strategies. Created with BioRender.com.
CRISPR, clustered regularly interspaced short palindromic repeats; dsDNA, double-stranded DNA; gRNA, guide RNA; ITR, inverted terminal repeat; mRNA, messenger RNA; RNP, ribonucleoprotein; ssDNA, single-stranded DNA; tCTS, truncated Cas9 target sequence.
