Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Apr 27;13:6874. doi: 10.1038/s41598-023-33932-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Characterization of DNMT1 knockdown cells (a) Relative increase in doxycycline-inducible sgRNA expression upon 24 h and 48 h hours of treatment with doxycycline. (b) Relative DNMT1 expression levels upon doxycycline treatment of DNMT1 knockdown cells. n = 4 per time point. (c) Western blot images of DNMT1 protein expression levels upon prolonged doxycycline treatment of DNMT1 knockdown cells for the indicated time points. (d) EPIC DNA methylation array analysis of DNMT1 knockdown cells cultured with or without doxycycline for 6 weeks. Top panels: heatmaps with methylation values for control (n = 3) and DNMT1 knockdown (n = 3) cells. Lower panels: Regional methylation profiles (composite plots) according to sample groups. DNMT1 knockdown cells consistently show reduced methylation density represented by lower β-values. For each region in the corresponding region type, relative coordinates of 0 and 1 correspond to the start and end coordinates of that region respectively. Coordinates smaller than 0 and greater than 1 denote flanking regions normalized by region length. Horizontal lines indicate region boundaries. (e) Violin plots of the β-values for genes, promoters and CGIs. Statistical test: paired Wilcoxon signed-rank test.