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. 2023 Apr 27;14:2425. doi: 10.1038/s41467-023-38118-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a (Left) RI images of an intact live U2OS cell. (Right) Combined RI and immunofluorescence images of the same cell after fixation. Anti-SRSF2 is used to target nuclear speckles. b (Top) Combined RI and fluorescence images of a live U2OS cell expressing EGFP-SRSF2. (Bottom left) Zoomed-in images of a single nuclear speckle. (Bottom right) Intensity profile plot for normalized fluorescence intensities of EGFP-SRSF2 (green line) and RIs (gray line) along the white dashed line. c (Left) RI images of a live U2OS cell treated with 500 μM sodium arsenite. (Right) Combined RI and immunofluorescence images of the same cell after fixation. Anti-G3BP1 is used to target stress granules. d (Top) Combined RI and fluorescence images of a live U2OS cell expressing G3BP1-EGFP after the treatment of 500 μM sodium arsenite. (Bottom left) Zoomed-in images of a single stress granule. (Bottom right) Intensity profile plot for normalized fluorescence intensities of G3BP1-EGFP (green line) and RIs (gray line) along the white dashed line. e (Left) Time-lapse images of a fusion event between two stress granules. (Right) Intensity profile plots for normalized fluorescence intensities of G3BP1-EGFP (green line) and RIs (gray line) along white dashed lines. All refractive index images are adjusted to 1.337–1.37.