Table 2.
Gene-specific primer sequences for gene expression of tight junction proteins and inflammatory cytokines in ileal tissues.
| Item1 | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|
| CLDN1 | GAAGATGCGGATGGCTGTCT | GGCCCGAGCCACTCTGTT |
| CLDN2 | CCCTGACAGCACCAAATTTGA | GCAGGAGGCACAGAGGATGA |
| CLDN3 | CGCGCTGCCCATGTG | GCTCTGCACCACGCAGTTC |
| CLDN4 | GGAGGACGAGACAGCCAAAG | GTTGGCCGACCAGCAGAT |
| OCLN | CATCGCCTCCATCGTCTACA | GTAGGCCTGGCTGCACATG |
| TJP1 | ACAAAAACAGAGCTGAACAACTAGCTA | GCTACGCAAACCTCGGAATC |
| MUC1 | CCTACCTGCCAGATACCATTGC | GAGAAGGGCTGGACTTGAGATG |
| TNF-α | TATGTGCAGCAACCCGTAGTG | CTGACTCATAGCAGAGACGTGTCA |
| IL-1β | ACCAACCCGACCAGGTCAA | ACATACGAGATGGAAACCAGCAA |
| IL-6 | CGGCCTGTTCGCCTTTC | CAGGTGCTTTGTGCTGTAGCA |
| CCL5 | CTGATACAACCGTGTGCTGCTT | GCTGCCTGTGGGCATTTG |
| ACTB | AACACCCACACCCCTGTGAT | TGAGTCAAGCGCCAAAAGAA |
1
CLDN1, claudin-1; CLDN2, claudin-2; CLDN3, claudin-3; CLDN4, claudin-4; OCLN, occludin; TJP1, tight junction protein-1; MUC1, mucin-1; TNF-α, tumor necrosis factor-α; IL-1β, interleukin-1β; IL-6, interleukin-6; CCL5, C–C motif chemokine ligand-5; ACTB, β-actin.