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. 2023 Apr 27;14:2413. doi: 10.1038/s41467-023-38100-9

Fig. 5. Allele-specific targeting of endogenous KRAS G12V RNA in a lung cancer cell model.

Fig. 5

a Schematic representation of the transcription and translation of allelic mutations associated with the expression of wild-type and G12V mutant KRAS proteins in NCI-H441 cells. b Schematic view of the PCR restriction fragment length polymorphism assay (PCR-RFLP) used to distinguish KRAS alleles associated with wild-type and mutant G12V protein expression. G-to-C mutation introduced by primer during PCR is shown in blue. The second position of codon 12 (boxed) encoding G12V or WT KRAS is shown in red. c Quantification of allele-specific G12V knockdown levels observed in NCI-H441 cells after 6, 12, and 24 h of treatment with either 50 or 500 nM Dz 10–23_v46 targeting KRAS G12V mRNA. Representative PCR-RFLP results are provided as an insert for each plot (n = 4). Error bars denote ± standard deviation of the mean for n = 4: 2 biological and 2 technical replicates. Source data are provided as a Source Data file.