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. 2023 Apr 14;14:1132265. doi: 10.3389/fimmu.2023.1132265

Figure 1.

Figure 1

C498 is identified as a dual-target inhibitor of STAT and NFκB signaling pathways by a luciferase drug screening system. (A), The chemical structure, molecular weight, and Canonical SMILES of C498. B-E, SKA-II, DU145 and A549 cells were seeded in 96-well plates and cultured overnight. Cells were treated with DMSO or a serial of C498 for 24 h. Luciferase activities of SKA-II cells were determined after 24 h (B). The cell viability of SKA-II (C), DU145 (D) and A549 (E) cells was determined after 24 h using resazurin assay. (F) HeLa and THP-1 cells were treated with DMSO or C498 at 2.5, 5, 10, and 15 μM for 2 h, followed by TNF-α stimulation (20 ng/ml, 10 min). Western blot analysis was determined on whole cell lysates and detected with anti-pSer176/180-IKKα/β antibodies. Tubulin was used as a loading control. (G) A549 and DU145 cells with constitutive STAT3 activation were treated with DMSO or C498 at 2.5, 5, 10, and 15 μM for 2 h. Western blot analysis was determined on whole cell lysates and detected with an anti-pTyr705-STAT3 antibody. Tubulin was used as a loading control.