Skip to main content
. 2023 Apr 14;14:1132265. doi: 10.3389/fimmu.2023.1132265

Figure 5.

Figure 5

C498 prevents temperature loss and tissue damage caused by LPS-induced septic shock in vivo. Mice were i.p. administrated with 5 mg/kg C498 12 h before the LPS challenge (6 mg/kg, i.p.). Body temperature (A) was recorded. Mice treated as in (A) were sacrificed 24 h after LPS injection and plasma ALT (B), AST (C), and BUN levels (D) were measured. Mice treated as in (A) were sacrificed 12 h after LPS injection and HE staining (100 ×) of inflammatory cell infiltration in the lung and kidney (E). Yellow arrows indicate neutrophil infiltration and black arrows indicate nuclei fragmentation. Mice treated as in (A) were sacrificed 12 h after LPS injection. Immunofluorescence (F, G) and relative fluorescence intensity (100 ×) of kidney and lung (H). The green color indicates CD11b staining and blue indicates nuclear DAPI staining (F-H). (I) The detection of 24 h plasma G-CSF levels by ELISA assay. *P < 0.05 is considered significant.