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. 2023 Apr 14;14:1132265. doi: 10.3389/fimmu.2023.1132265

Figure 6.

Figure 6

C498 inhibits the expression of relevant inflammatory factors in sepsis models. Mice were i.p. administrated with 5 mg/kg C498 12 h before the LPS challenge (6 mg/kg, i.p.) and sacrificed at 12h or 24h after LPS injection. The detection of 24 h plasma IL-6 levels by ELISA assay (A). Mice treated as in (A) were sacrificed 12 h after LPS injection and the relative mRNA levels of IL-6 in kidney (B) and lung (C) were determined by RT- PCR. Immunofluorescence (D, E) and relative fluorescence intensity (F) of kidney and lung (100 ×). Red color indicates IL-6 staining and blue indicates nuclear DAPI staining (D-F). The detection of 12 h plasma IL-1β levels (G) by ELISA assay and the relative mRNA levels of IL-1β in kidney (H) by RT- PCR. The mRNA levels of TNF-α (I, J), CCL3 (K, L), IL-17A (M, N), CXCL1 (O, P), IFN-γ (Q), and IFN-β (R) within kidney (I, K, M, O, Q) and lung (J, L, N, P, R) tissues were determined by RT- PCR. *P < 0.05 is considered significant.