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. 2023 Apr 14;14:1139725. doi: 10.3389/fendo.2023.1139725

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Copyright © 2023 Yuan, Zhang, Wang, Ma, Liu, Zhao, Lu and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Intracellular ROS levels were measured by assessing DCFH fluorescence (A–F). Quantitative analysis showed that the ROS levels of four- and eight-cell embryos were significantly decreased (P < 0.05) in the FOVE group compared with the control group (C–F). There were no significant difference in ATP content between two- and four-cell shown in (G–J) (P > 0.05). As shown in Figures 2K, L, the ATP levels of eight-cell embryos were significantly higher in the FOVE group than in the control group (P < 0.05).