Table 4.
Frozen-thawed semen plasma antioxidant status and hormones.
| Control | FO | VE | FOVE | overall | |
|---|---|---|---|---|---|
| CAT | |||||
| 0 week | 187.67 ± 31.81 | 194.32 ± 20.57 | 192.65 ± 30.21 | 182.69 ± 25.16 | 189.33 ± 24.86b |
| 4 week | 181.03 ± 46.79 | 174.38 ± 23.26 | 177.7 ± 23.26 | 192.65 ± 28.71 | 181.44 ± 29.49b |
| 8 week #$ | 187.67 ± 9.97 | 240.83 ± 19.84 | 249.13 ± 27.4 | 252.46 ± 12.13 | 232.52 ± 31.82a |
| 10 week #$ | 139.5 ± 19.56 | 177.7 ± 23.88 | 182.69 ± 8.58 | 200.96 ± 9.97 | 175.21 ± 27.54b |
| overall | 173.97 ± 34.16 | 196.81 ± 33.7 | 200.55 ± 36.41 | 207.19 ± 33.38 | – |
| GSH-Px | |||||
| 0 week | 431.54 ± 32.9 | 448.73 ± 36.51 | 402.24 ± 44.73 | 451.6 ± 16.13 | 433.53 ± 36.69b |
| 4 week | 420.39 ± 27.17 | 461.15 ± 41.98 | 466.88 ± 68.15 | 479.62 ± 30.69 | 457.01 ± 46.28b |
| 8 week | 577.39 ± 43.67 | 611.47 ± 27.84 | 543.95 ± 51.65 | 589.18 ± 51.59 | 580.5 ± 47.3a |
| 10 week # | 339.82 ± 15.05 | 377.08 ± 64.77 | 430.58 ± 17.85 | 467.2 ± 24.38 | 403.67 ± 60.1b |
| overall | 442.28 ± 92.83 | 474.61 ± 96.81 | 460.91 ± 70.27 | 496.9 ± 63.44 | – |
| SOD | |||||
| 0 week | 42.78 ± 5.13 | 43.15 ± 1.61 | 42.44 ± 2.1 | 40.88 ± 8.73 | 42.31 ± 4.76b |
| 4 week | 44.04 ± 2.68 | 44.86 ± 4.5 | 45.08 ± 3.52 | 49.35 ± 2.27 | 45.83 ± 3.68b |
| 8 week $ | 49.98 ± 7.22 | 57.74 ± 6.43 | 58.74 ± 8.98 | 58.89 ± 3.04 | 56.34 ± 7.16a |
| 10 week | 38.99 ± 4.91 | 44.37 ± 3.03 | 41.7 ± 2.29 | 44.56 ± 1.7 | 42.41 ± 3.72b |
| overall | 43.95 ± 6.21 | 47.53 ± 7.22 | 46.99 ± 8.44 | 48.42 ± 8.2 | – |
| MDA | |||||
| 0 week | 12.71 ± 3.92 | 13.95 ± 3.87 | 14.15 ± 2.8 | 13.99 ± 2.18 | 13.7 ± 2.99b |
| 4 week | 14.41 ± 1.52 | 13.36 ± 1.56 | 13.78 ± 0.68 | 10.05 ± 3.12 | 12.9 ± 2.45b |
| 8 week $ | 17.51 ± 3.83 | 17.21 ± 2.44 | 16.71 ± 1.9 | 13.04 ± 1.11 | 16.12 ± 2.92a |
| 10 week # | 13.45 ± 2.62 | 7.55 ± 0.79 | 8.79 ± 0.89 | 8.92 ± 2.21 | 9.68 ± 2.83c |
| overall | 14.52 ± 3.38 | 13.02 ± 4.21 | 13.36 ± 3.36 | 11.5 ± 2.96 | – |
| ROS | |||||
| 0 week | 126.86 ± 25.94 | 130.56 ± 13.73 | 129.82 ± 27.3 | 131.02 ± 12.53 | 129.56 ± 18.86b |
| 4 week | 123.08 ± 16.84 | 127.23 ± 9.42 | 139.05 ± 11.17 | 146.81 ± 12.85 | 134.04 ± 15.07b |
| 8 week #$ | 204.72 ± 21.8 | 176.92 ± 19.98 | 159.83 ± 11.32 | 156.97 ± 8.05 | 174.61 ± 24.45a |
| 10 week #$ | 131.39 ± 4.4 | 93.34 ± 6.34 | 78.65 ± 11.04 | 85.58 ± 4.21 | 97.24 ± 21.98c |
| overall | 146.51 ± 38.78 | 132.01 ± 32.96 | 126.84 ± 34.29 | 130.1 ± 29.61 | – |
| T (nmol/L) | |||||
| 0 week | 15.28 ± 1.46 | 13.99 ± 1.22 | 14.54 ± 0.55 | 15.28 ± 1.14 | 14.77 ± 1.16b |
| 4 week # | 15.92 ± 0.98 | 17.68 ± 0.97 | 16.66 ± 2.01 | 19.61 ± 1.43 | 17.47 ± 1.91b |
| 8 week #$ | 19.7 ± 0.46 | 22.1 ± 2.73 | 21.36 ± 2.12 | 25.33 ± 1.09 | 22.13 ± 2.67a |
| 10 week # | 9.37 ± 1.42 | 14.26 ± 0.6 | 9.93 ± 0.18 | 13.99 ± 0.55 | 11.89 ± 2.44c |
| overall | 15.07 ± 3.95 | 17.01 ± 3.68 | 15.62 ± 4.45 | 18.55 ± 4.68 | – |
| E2 (pmol/L) | |||||
| 0 week | 232.06 ± 28.15 | 212.07 ± 30.18 | 238.91 ± 36.85 | 222.62 ± 19.8 | 226.41 ± 28.28c |
| 4 week # | 226.59 ± 10.17 | 269.16 ± 12.26 | 217.82 ± 7.76 | 278.88 ± 18.09 | 248.11 ± 29.45b |
| 8 week # | 264.78 ± 27.96 | 299.42 ± 54.78 | 292.71 ± 19.57 | 317.77 ± 44.5 | 293.67 ± 40.19a |
| 10 week # | 113.64 ± 12.68 | 129.24 ± 22.32 | 116.79 ± 14.21 | 135.82 ± 4.78 | 123.87 ± 16.24d |
| overall | 209.27 ± 62.02 | 227.48 ± 73.43 | 216.56 ± 68.81 | 238.77 ± 74.39 | – |
Data shown are the mean ± SD (n = 4 replicates per treatment, one ejaculate per replicate). For each parameter, values within columns with different superscript letters differ significantly (P < 0.05). #Significant (P < 0.05) main effect of flaxseed oil (FO). $Significant (P < 0.05) main effect of vitamin E (VE).